|
Table S1. Sample Details for Each Proteomic Experiment |
||||
|
Sample |
Media |
Yeast Strain |
Purification |
YDPM File Nameb |
|
A |
YP-Lactate |
W303 |
DG |
yplDG1_matY |
|
B |
YP-Lactate |
W303 |
DG |
yplDG1_memY |
|
C |
YP-Lactate |
W303 |
DG |
yplDG2_memY |
|
D |
YP-Lactate |
W303 |
DG |
yplDG3_memY |
|
1 |
YP-Lactate |
W303 |
FFE |
ypl01_memY ypl01_memY_FT ypl01_matY ypl01_matY_FT |
|
2 |
YP-Lactate |
W303 |
FFE |
ypl02_memY ypl02_memY_FT ypl02_matY ypl02_matY_FT |
|
4 |
YP-Dextrose |
W303 |
FFE |
ypd04_memY ypd04_matY |
|
5, 10, 11a |
SC-Lactate |
S1001 |
FFE |
scl01_memY scl01_memY_FT scl01_matY scl01_matY_FT |
|
7, 12a |
SC-Dextrose |
S1001 |
FFE |
scd01_memY scd01_memY_FT scd01_matY scd01_matY_FT |
|
13 |
YP-Dextrose |
W303 |
FFE |
ypd13_memY ypd13_matY |
|
14 |
YP-Lactate |
W303 |
FFE |
ypl14_memY ypl14_matY |
|
15 |
YP-Lactate |
W303 |
FFE |
ypl15_memY ypl15_matY |
aIn rows with multiple samples, more than one culture was grown under the same conditions (same media, same strain), but mitochondria were purified separately. Due to low protein yield, corresponding fractions were joined for MS analysis.
bDatasets produced by MS: filenames ending with FT were derived from FTICR analysis, all others from LC/MS/MS. Filenames containing ³mem² designate analysis of the mitochondrial membrane fraction; ³mat,² of the mitochondrial matrix fraction. The datasets and more information about them are available at http://www-deletion.stanford.edu/YDPM/YDPM_index.html.
YP, yeast peptone; SC, synthetic complete; DG, differential centrifugation by Nycodenz density gradient; FFE, free-flow electrophoresis.
DOI: 10.1371/journal.pbio.0020160.t001